PCR chubu
1.Used yaing'ono batch nyemba amveke mayankho anachita, angagwiritsidwe ntchito limodzi kapena ulendo. Machubu a PCOR a CORBITION amapangidwa kuchokera ku UPS level polypropylene. Machubu onse ndi ovomerezeka ndi muyezo 96 wa mbale. Kapangidwe ka khoma locheperako kuti kutentha kutengeke bwino. Tight kusindikiza kapu kuteteza nyemba evaporation. Machubu amapezeka mosiyanasiyana mosiyanasiyana posungira zitsanzo.
0,1 ml, 0,2 ml, umodzi ndi 8 Mzere, mandala ndi oyera mitundu, zipewa mosabisa ndi zisoti olamulira akhoza kukhala options. 0.1 ml machubu amapezeka oyera mopepuka kuti awonjezere chizindikiro pakuyesa kwamphamvu. PCR chubu ndi zinthu zabwino kwa PCR yachangu komanso kutsika kwa voliyumu. Ma machubu onse ndi opanda DNase, opanda RNase, osakhala a pyrogenic.
2. Njira yolamulira ya Octet PCR
Ubwino wazinthuzo sizingafanane ndi momwe zimapangidwira. MwaukadauloZida luso kupanga ndi zida mkulu mwatsatanetsatane ndi chiyembekezo cha mankhwala. Kuyesera kwa PCR kumayang'anitsitsa kukhoma kwa PCR zakuthupi, ndipo pamafunika kukhazikika kwa khoma ndi yunifolomu pachilichonse kuti muwonetsetse gawo lotentha. Kutentha kunasamutsidwa mofananamo kuzitsanzo za octuplets a PCR kuti akwaniritse kulondola kwa zotsatira zoyeserera.
3, yowonongeka ndi kuwala,
Chifukwa cha zida za PCR zochulukirapo za fulorosenti, njira yopepuka imayenera kuyendetsedwa kuchokera pamwamba pazogulira mpaka mkati mwa chubu, kotero kuwonetsetsa kowonekera kwa zomwe zikuwonongeka kuyenera kukhala kwakukulu kwambiri.
4, kusindikiza
Kusindikiza kosindikiza kwa chubu ndi chubu cha thupi kumayenera kukhala zabwino makamaka popewa kutuluka kwa madzi mu chubu, pewani kuwonongeka pamtanda, ndikuwonetsetsa kulondola kwa zotsatira zoyeserera!
5. Malo opangira
Ndi zida zabwino, malo ochitira misonkhano yoyera ndiyofunikanso kwambiri. Ngati chitsanzocho chidetsedwa ndi zakuda, zotsatira zake sizikhala zolondola kwambiri. Zomwe zimafufuzidwa ndizowononga nthawi komanso zimakhala zotopetsa. Kuyendetsa bwino zinthu pakapangidwe kazinthu kumatha kuonetsetsa kuti gulu lililonse lazogulitsa likugwira ntchito. Khola kuti liwonetsetse kulondola ndi kubwereza kwa chidziwitso choyesera
1.Selection wa chilungamo kwambiri kunja zinthu polypropylene zakuthupi, palibe mpweya.
2. Palibe michere ya DNA kapena RNA.
3.The kopitilira muyeso-woonda yunifolomu chubu khoma anazindikira mwa mwatsatanetsatane mamangidwe nkhungu. Khoma la chubu yunifolomu yopyapyala kwambiri imapereka kutentha kopitilira muyeso ndikulimbikitsa kukulitsa kwa kukweza kwa zitsanzo.
4. Tsekani mwamphamvu chitoliro kuti chitetezeke.
5.Zogulitsazo ndizoyenera pazida zambiri za PCR zamagetsi.
6.Made kuchokera UPS Pulasitiki Maphunziro VI Polypropylene.
7.Yogwirizana ndi mbale yokhazikika ya 96
8. Khoma laling'onoting'ono lakusinthira kutentha kumapezeka poyera komanso yoyera, kapu yosalala ndi kapu yoyenda
9.Tight wosindikiza kapu kapangidwe kuteteza nyemba evaporation
10.Sterile kufikira SAL mulingo 10-6
| Ayi. | Kufotokozera | Voliyumu | Kapu | Kufotokozera | Kulongedza | Yolera yotseketsa |
| Zogwirizana | PCR chubu | 0.2ml | Lathyathyathya kapena Dome | Osakwatira | 20000 / Ctns | zosankha |
| Chombo001 | 8-chabwino PCR Mzere | 0.2ml | Lathyathyathya | 8 Mzere | 5000 / Ctns | zosankha |
| Chombo | Yaying'ono PCR chubu | 25ul | Lathyathyathya | Osakwatira | 12000 / Ma Ctns | zosankha |
| Chombo | 8-chabwino PCR Mzere | 0.1ml | Lathyathyathya | 8 Mzere | 5000 / Ctns | zosankha |
| Chombo | PCR chubu | 0.1ml | Lathyathyathya | Osakwatira | 2500 / thumba kapena 10000 / thumba | zosankha |
PC0021 0.2ml 8 Mzere PCR chubu |
PC0050 25ul yaying'ono PCR chubu |
PC0051 0.1ml 8 Mzere PCR chubu |
|
PC1001 0.2ml PCR chubu mosabisa & mzikiti kapu |
